In the rapidly evolving landscape of drug discovery, the identification and validation of novel therapeutic targets are pivotal to expanding the druggable genome and tackling unmet medical needs. This forum focuses on novel targets and the cutting-edge methodologies and technological innovations that are greatly advancing the tractable target space. The forum will bring together leading academic and industry experts to reflect on the latest approaches, opportunities and challenges to exploit historically challenging and novel target classes using state-of-the-art methodologies.