Event Overview: The 9th biennial High Content Imaging and Flow Cytometry in Drug Discovery 2025 will be held at GSK Medicines Research Centre, Stevenage. Flow cytometry and imaging are two powerful technology platforms that enable high-content analysis of complex cell systems providing invaluable insights in drug discovery and clinical evaluations. Since our first meeting in 2008, we have seen the technology develop and the applications widen across many areas of industrial and academic drug discovery and development. The integration of advanced machine learning and AI techniques is revolutionizing the way researchers harness and interpret data from these high-content platforms, further accelerating the pace of innovation in the field.